Sequencing Grade trypsin

Sequencing Grade Modified Trypsin

1.DESCRIPTION
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant  trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

2.MAIN FEATURES
Source           Recombinant E. coli

Appearance   White or off white, or yellowish powder

Specific activity≥4500 USP units/mg pro.

SDS-PAGE             Single major band

Molecular Weight(SDS-PAGE)    24.0±2.4 kDa

Purity(HPLC)     ≥95%

 
3.RECOMMEND USAGE
It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO₃ or ph7.0-8.0 buffer solution. 1 mM CaCl₂ is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

 
4.PRODUCT USAGE
The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.