sequencing grade human chymotrypsin

sequencing grade human chymotrypsin
Product Description

DESCRIPTION
Sequencing grade chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Sequencing grade chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. The optimal pH is 7.0–9.0. 
PRODUCT USAGE
Sequencing grade chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.


Shanghai Yaxin Biotechnology Co.,Ltd

  • CN
  • 2015
    On CPHI since
Specifications
  • Details
    Lipases (EC 3.1.1.3) are ubiquitous enzymes that catalyze the hydrolysis of fats and oils, playing important roles in pharmaceutical. The lipase from Serratia marcescens (SML) is well-known for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM], a key intermediate for the synthesis of diltiazem hydrochloride.

    SOURCE: E. coli;
    ENZYME COMMISSION NUMBER: 3.1.1.3;
    SEQUENCE: Lipase A gene from Serratia marcescens.
    COMPONENTS: It is a natural immobilized lipase A. No other additivies.
    UNIT DEFIINITION: Lipase was assayed using p-nitrophenyl acetate ( pNPA) as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 µmol of p-nitrophenol per minute.
    USAGE METHODS: Used directly after suspended, remove this insoluble lipase by centrifugation, and can be reused several times.
    STORAGE CONDITIONS: -2
  • Selling Points
    Quality Service; Recombinant Products of high purity; Animal component-free which is convenient and safe.
  • Supplied from
    China
  • Measured In
    gram; milligram

Shanghai Yaxin Biotechnology Co.,Ltd

  • CN
  • 2015
    On CPHI since

More Products from Shanghai Yaxin Biotechnology Co.,Ltd (24)

  • Recombinant Protein A

    Product Recombinant Protein A

    DESCRIPTION
    Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies...
  • Human Chymotrypsin enzyme

    Product Human Chymotrypsin enzyme

    DESCRIPTION
    Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but a...
  • Recombinant Trypsin Inhibitor

    Product Recombinant Trypsin Inhibitor

    DESCRIPTION
    Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH <3.0. E...
  • Sequencing Grade Carboxypeptidase B

    Product Sequencing Grade Carboxypeptidase B

    DESCRIPTION
    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from  the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysin...
  • Sequencing Grade  trypsin

    Product Sequencing Grade trypsin

    Sequencing Grade Modified Trypsin

    1.DESCRIPTION
    YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other protease...
  • Sequencing Grade V8 Protease

    Product Sequencing Grade V8 Protease

    DESCRIPTION    
    Recombinant V8 protease is the serine protease family and can specifically hydrolyzing glutamic acid or aspartic acid residues carboxy side peptide bonds. The Glu carboxy terminal peptide bond can be identified and cleaved in CH4COONH4 buffer (pH 7.8)&nb...
  • Kex 2 protease

    Product Kex 2 protease

    DESCRIPTION
    Kex2 is a Ca2+ dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Ke...
  • Recombinant Porcine Trypsin

    Product Recombinant Porcine Trypsin

    Source                      Recombinant E. coli

    Appearance           White or off white, or yellowish powder

    Specific activity   ≥3800 USP units/mg pro.

    Purity(SDS-...
  • Recombinant Porcine Trypsin

    Product Recombinant Porcine Trypsin

    Trypsin is a member of the serine protease family. Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid residues. The pH optimum of trypsin is pH7 - 10. The enzyme is inhibited by serine proteases inhibitors, e.g. PMSF, and metal
    chelating agents, e.g., EDTA. Recombinant...
  • Recombinant Porcine Trypsin enzyme

    Product Recombinant Porcine Trypsin enzyme

    Trypsin is a member of the serine protease family. Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid residues. The pH optimum of trypsin is pH7 - 10. The enzyme is inhibited by serine proteases inhibitors, e.g. PMSF, and metal
    chelating agents, e.g., EDTA. Recombinant...
  • Recombinant Protein A

    Product Recombinant Protein A

    The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
  • Recombinant Trypsin solution

    Product Recombinant Trypsin solution

    DESCRIPTION
    Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human ...

Frequently Viewed Together

Recently Visited