Recombinant Aprotinin

Product Description

DESCRIPTION
Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH <3.0. Effective concentration is equimolar with protease. YaxinBio recombinant aprotinin is a genetically engineered protein expressed in E.coli and purified by multi-column.The amino acid sequence of recombinant trypsin is identical to bovine lung derived aprotinin,with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes.
PRODUCT USAGE
Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Recombinant aprotinin is with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes,such as inhibition of serine protease activity in recombinant protein production,cell culture and so on.

Shanghai Yaxin Biotechnology Co.,Ltd

CN
2015

On CPHI since

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Specifications

Details

Lipases (EC 3.1.1.3) are ubiquitous enzymes that catalyze the hydrolysis of fats and oils, playing important roles in pharmaceutical. The lipase from Serratia marcescens (SML) is well-known for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM], which is a key intermediate for the synthesis of diltiazem hydrochloride.

SOURCE: E. coli;
ENZYME COMMISSION NUMBER: 3.1.1.3;
SEQUENCE: Lipase A gene from Serratia marcescens.
COMPONENTS: It is a natural immobilized lipase A. No other additivies.
UNIT DEFIINITION: Lipase was assayed using p-nitrophenyl acetate (pNPA) as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 µmol of p-nitrophenol per minute.
USAGE METHODS: Used directly after suspended, remove this insoluble lipase by centrifugation, and can be reused several times.
STORAGE CONDIT
Selling Points
Quality Service; Recombinant Products of high purity; Animal component-free which is convenient and safe.
Supplied from

China
Measured In
gram