Acetic Acid Glacial

Glacial Acetic Acid BP Grade Ph Eur -- C2H4O2 -- 60.1 -- 64-19-7

DEFINITION
Content: 99.0 per cent m/m to 100.5 per cent m/m.
CHARACTERS
Appearance: Crystalline mass or clear, colourless, volatile liquid.
Solubility: Miscible with water, with ethanol (96 per cent) and with methylene chloride.
IDENTIFICATION
A. A 100 g/L solution is strongly acid (2.2.4).
B. To 0.03 mL add 3 mL of water R and neutralise with dilute sodium hydroxide  solution R. The solution gives reaction (b) of acetates (2.3.1).
TESTS
Solution S: Dilute 20 mL to 100 mL with distilled water.
Appearance: The substance to be examined is clear and colourless.
Freezing point: Minimum 14.8C.
Reducing substances: To 5.0 mL add 10.0 mL of water and mix. To 5.0 mL of this solution add 6 mL of sulfuric acid, cool and add 2.0 mL of 0.0167 M potassium dichromate. Allow to  stand for 1 min and add 25 mL of water and 1 mL of a freshly prepared 100 g/L  solution of potassium iodide. Titrate with 0.1 M sodium thiosulfate, using 1.0 mL of  starch solution as indicator. Not less than 1.0 mL of 0.1 M sodium thiosulfate  solution is required.
Chlorides: Maximum 25 mg/L.
Sulfates: Maximum 50 mg/L, determined on solution S.
Iron: Maximum 5 ppm.
Heavy metals:  Maximum 5 ppm.
Residue on evaporation: Maximum 0.01 per cent.
Evaporate 20 g to dryness on a water-bath and dry at 100-105 °C. The residue  weighs a maximum of 2.0 mg.
ASSAY: Weigh accurately a conical flask with a ground-glass stopper containing 25 mL of  water R. Add 1.0 mL of the substance to be examined and weigh again accurately.  Add 0.5 mL of phenolphthalein solution R and titrate with 1 M sodium hydroxide.
1 mL of 1 M sodium hydroxide is equivalent to 60.1 mg of C2H4O2.

Glacial Acetic Acid USP Grade
C2H4O2 60.05
Acetic acid -- 64-19-7.
Glacial Acetic Acid contains not less than 99.5 percent and not more than 100.5 percent, by weight, of C2H4O2.

Identification: A mixture of 1 volume of it with 2 volumes of water responds to the tests for Acetate.
Congealing temperature: not lower than 15.6 .
Limit of nonvolatile residue: Evaporate 20 mL in a tared dish, and dry at 105 for 1 hour: the weight of the residue does not exceed 1.0 mg.
Chloride: Dilute 1.0 mL with 20 mL of water, and add 5 drops of silver nitrate. No opalescence is produced.
Sulfate: Dilute 1.0 mL with 10 mL of water, and add 1 mL of barium chloride. No turbidity is produced.
Heavy metals: To the residue obtained in the test for Limit of nonvolatile residue add 8 mL of 0.1 N hydrochloric acid, warm gently until solution is complete, dilute with water to 100 mL, and use 20 mL of the solution: the limit is 5 ppm.
Readily oxidizable substances: Dilute 2.0 mL in a glass-stoppered vessel with 10 mL of water, and add 0.10 mL of 0.10 N potassium permanganate: the pink color is not changed to brown within 2 hours.