Heparan sulfate antibodies applications guide

New 24-page guide that brings together applications, protocols, results and citations for researchers.

Heparan Sulfate (HS) is synthesized as the glycosaminoglycan (GAG) component of heparan sulfate proteoglycans (HSPGs). It is expressed on the cell surface of virtually all cell types and basement membranes. It displays specific interactions with many biologically active proteins and, thus is involved in many important biological processes. Many researchers are interested in examining the dynamic distribution of HSPG in tissues and also analyzing HS structures to elucidate the biological functions of HSPG. The non-immunogenic character of HS makes this type of antibody difficult to raise, so the few hybridoma-derived mouse anti-HS antibodies such as JM403, 10E4 and 3G10 are valuable tools for HS research. Using multiple HS antibodies that recognize subtle differences in HS patterning can be useful in applications such as flow cytometry allowing multiple cell types to be directly compared.

Amsbio offers a suite of high quality heparan sulfate antibodies from the important clones F69-3G10, F58-10E4 and JM403. These antibodies are ideal for targeted binding of HS for HSPG research and provide a range of anti-HS antibodies recognizing distinct HS substructures, which have been well-characterized in previous studies. The guide provides an informative introduction to applications of JM403, 10E4 and 3G10 Heparan Sulfate antibodies; including use of the 10E4 and JM-403 clones together in ELISA to detect or measure HS. GAGs are not species-specific, so in common with AMSBIO’s other GAG antibodies, the clones featured in this guide react across a wide range of species.

Johan van der Vlag, Associate Professor at Radboud University Medical Center, Nijmegen commented: “I am extremely happy to see that our JM403 clone is commercially available to serve the scientific community that studies the role of GAGs in normal physiology and pathology. Since we only want to licence our antibody to a company that is dedicated to glycobiology and in particular GAGs, AMSBIO was our only choice.”